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Pyrosequencing: Innovations and Applications

Prerna Pandey

9781773611501
297 pages
Arcler Education Inc
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Overview
Pyrosequencing is a DNA sequencing technique that involves sequencing by synthesis, and employs a series of enzymes to detect nucleic acid sequences during the synthesis and enables qualitative analysis of the DNA. A major advantage of this technique is that the labeled primers and nucleotides, as well as gel-electrophoresis is not required. The method requires minimal amounts of samples and gives a high throughput at a lower cost. Besides, the sequence reaction time per base calling is shortened, with higher accuracy, better automation and parallel processing. Pyrosequencing offers a valuable tool for obtaining and analysis of accurate data, identification of new alleles, polymorphic genetic markers, especially Single Nucleotide polymorphisms.The primary purpose of this book is to discuss basics of the Pyrosequencing technology and its key features, including the general sequencing modes, limitations, and future applications. We look forward to feedback by our readers
Author Bio
Dr Prerna Pandey, a biotechnologist with several years of wet lab research experience, has worked as a senior research fellow at International Center for Genetic Engineering and Biotechnology, New Delhi. Her field of research in PhD included isolation and molecular characterization of Geminiviruses, genome sequencing, gene annotation, as well as gene silencing using the RNA interference technology. She has published papers in peer-reviewed journals in the field and has a submitted a number of annotated Geminiviral genome sequences in the GenBank, including two novel ones. Prerna has also completed her editing and proof-reading courses from SfEP, UK and now works as a freelance scientific editor, writer and a subject matter expert. She has edited and co-authored several books on a range of topics in Life-Sciences. When Prerna is not busy with her assignments, she loves visiting historical places.

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