Overview of blotting techniques
Shiv Sanjeevi Sripathi
Prerna Pandey
9781774076958
pages
Arcler Education Inc
Overview
There are 2 techniques that are commonly employed in research labs: gel electrophoresis and blotting. Following electrophoresis, separated nucleic acids and proteins can be transferred to a membrane where they are analyzed or detected. Depending on the molecule being analyzed, Southern blot refers to transfer and studying DNA, Northern blotting refers to RNA, Western blotting to proteins and eastern blotting for post-translational modifications. Given the importance of blotting in the lab, this book comes at an opportune time. The book discusses the protocols and applications of blotting-Southern, western, northern and eastern blotting. Case studies have also presented to help the reader gain insights into the practicality and applicability of blotting in today’s world. The editors hope that this book will serve as a lab staple just like the aforementioned blotting techniques.
Author Bio
ShivSanjeevi Sripathi completed his Masters in Biotechnology from Mumbai University in 2008. He was awarded for academic excellence in both his Bachelors and Masters for securing second rank in Mumbai University in 2006 and first rank in his college: Kishinchand chellaram College. For his Masters he secured first rank in his college KET's V.G.Vaze College. He qualified CSIR and NET and and TOEFL in September 2008. He then worked on a stem cell project at the Specialized Centre for Cell Based Therapy (SCCT),KEM Hospital at Mumbai on a project entitled, : Isolation & detection of stem cells from Human Umbilical cord/ amniotic membrane" following which he worked at Junior Research Fellow at Microbiology & Cell Biology Department, Indian Institute of Science, Bangalore from August 2009 to November 2011. The field of work involved cloning of cell wall genes and transcription factors in E.coli & M.smegmatis. As a writer, he has authored and co-authored 25 books with Delve and Arcler International Press. He loves to read and share on interesting aspects of life sciences in books. In his free time he loves to travel and explore ancient customs and traditions.
